OPTIMIZATION OF THE NUTRIENT MEDIUM FOR THE PRIMARY STAGES OF COMMON WALNUT MICROCLONAL PROPAGATION IN VITRO

SummaryAim. To optimize the processes of microclonal propagation Juglans regia in vitro by selecting composition and consistency nutrient medium. Methods. The methods culture establishment initial explants were used. Parts stem buds young sprouts, germinated mini-greenhouse method from seeds regia, used as explants. Murashige&Skoog (MS) Driver&Kuniyuki (DKW) media to establish vitro. Agar was a gelling agent. solid, semi-liquid, liquid prepared with addition 7 g/l, 3.5 g/l 0 agar, respectively. All experimental variants modified adding phytohormone cytokinin group 6-BAP. pH medium controlled at level 7.1–7.2. established cultivated temperature + 25°C, light intensity 2500 lux, relative humidity 56–70% photoperiod 16/8 h light/dark. On 3rd, 7th 14th day, survival rate, growth development parameters estimated. Results. It found that optimal for cultivation is DKW medium, on which high rate observed (80%), well accelerated swelling their proliferation. proposed use semi-liquid stages common walnut micropropagation contributed acceleration reproduction this 2 days improved 20% compared solid Conclusion. introduction into (Juglans regia) recommended increase 20%, activation (2 days) further